Michael Sanchez, the brother of Jeff Bezos’ girlfriend, is alleging in a lawsuit that the publisher of the National Enquirer defamed him when it claimed that he was the source of the tabloid’s 2019 exposé about the Amazon.com Inc. founder’s extramarital affair.

The lawsuit, filed Friday in federal court in Los Angeles, centers around the publication of a January 2019 article in the National Enquirer exposing an affair between Mr. Bezos and Lauren Sanchez, Mr. Sanchez’s sister, both of whom were married at the time. Mr. Bezos…

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